Frequently Asked Questions

Please browse through these FAQs to find answers to the most commonly raised questions.

Can’t find what you are looking for? Contact our technical support team.

Installation questions

General instructions:

Remove any previous stage and ensure there is maximum clearance for the stage, i.e., lower (raise) the condenser and if required remove the objectives. Unpack the Prior stage from the box and ensure you place the stage in a wide space. Turn the stage so that the Prior logo is at the front right (next to your right hand as you hold the stage). Place the stage onto dovetail (substage) and secure the stage. Please see below for different ways to secure your stage.

For dovetail fixings, you will be required to tighten a screw to fix the stage (some are spring-loaded and require pulling back to fit the stage).

For screw fittings, you will have to move the stage to reveal the mounting holes. Before trying to move the stage ensure the transit bolts are taken off. Hold the stage so that it aligns with the holes on the dovetail with one hand while you fix several screws to hold it in position. Ensure you have fitted all the screws before tightening each of them in turn.

Push the stage (if possible) through its travel range to ensure no collisions with any microscope fittings. If required adjust the limit switches provided on the stage to limit the travel range. Use changing limits to change the limits of travel on the stage.

You can download detailed instructions to change the limits of your stage so that it no longer collides with the microscope:

H117, H101A and ES111 Stage Limit Adjustment

If adjusting the limits doesn’t resolve the problem, please report your stage model and microscope make and model and a description of the problem to technical support.

Ensure you have the correct adapter for the microscope and position on the microscope: please contact us for the relevant manual. The adapter part number will be on your dispatch note and on the packaging. Generally, there are two positions in which the filter wheel could be fitted:

  1. Emission: the filter wheel will fit between the sample and the camera port or/and eyepieces.
  2. Excitation: the filter wheel will fit between the excitation (Hg, Lumen, or other light source) and the microscope.

The filter wheel is normally installed with the motor pointing away from the microscope. Look at the parts of the adapter: one will mirror the connection on the microscope female flange, the other will fix to it male flange.

You should attach the female flange to the side of the filter wheel with the motor on it, and male flange to the flat side of the filter wheel. In some cases you may need to remove the filter wheel cover to attach the adapter.

Filter rings and a filter change tool are supplied with your filter wheel.

The document below explains how to install filters in detail:

Changing Filters on the Filter Wheels

 

Ensure you have the correct adapter for the microscope and have located the correct position on the microscope.

The adapter part number will be on your dispatch note and on the packaging. Generally there are three positions which the shutter could be fitted:

  1. Bright field: the shutter will fit between the bright field light source and the sample
  2. Emission: the shutter will fit between the sample and the camera port or/and eye pieces.
  3. Excitation: the shutter will fit between the excitation (Hg, Lumen, or other light source) and the microscope.

The shutter is normally installed with the silver side of the shutter blades pointing towards the light source.

Look at the parts of the adapter. One will mirror the connection on the microscope female flange, the other will fix to it the male flange. You should attach the female flange to the side of the shutter with the black blades and male flange to the side of the shutter with the silver blades. In some cases you may need to remove the shutter cover to attach the adapter.

Standard focus motors can be installed onto most microscopes with a coarse and fine focus knob. The focus can be installed on the left or right side of the microscope. The direction of turn of the motor can be reversed in the controller to compensate: see the manual for your controller for RS232 commands.

Place the focus sleeve over the coarse focus, place the focus housing over the sleeve and fix with the grub screws. Push the motor into the focus housing and gently push the pad onto the fine focus, fix the motor in position with the screw.

Visit the download section for installation manuals for direct couplings

There are two different bulb fitting procedures. If you purchased your unit before August 2007 please use this guide:

Lumen 200 Bulb Installation (Old variants)

If you purchased your Lumen after August 2007 please use this guide:

Bulb Installation for the Lumen 200 Series

Important: Ensure your Lumen 200 is installed with adequate airflow around it.

 

Before attempting to install a controller, visit the download section and choose the relevant manual to download. Ensure that the power supply to the controller AND the accessories are switched off before you attempt to connect them.

The plastic screws help ensure that your sample holder is held firmly in the stage. They also guarantee that your sample moves the proper amount and is registered properly with the stage when performing sensitive experiments. The screws should not be over-tightened, but loosely threaded into the holes. The nylon material also allows for tilting of the holder when the leveling grub screws are adjusted.

Support questions

Yes, the Lumen 220 model is available for Cy5.5.

Yes, the standard Lumen 200 wavelength range is sufficient to cover both CY5 and DAPI.

Yes, we offer a service for larger quantities of light sources where we will design light sources for the purposes of our customers. Please see the OEM section of the website for more information on this, or contact technical support.

An encoded focus is required if the microscope being used has a focus mechanism that is not directly connected to the fine focus system and, therefore, when fine focus is moved the course focus moves as well. This includes most Zeiss models. In this case a Z-encoder is recommended to provide a reliable Z-position.

An encoder kit is required for most Zeiss microscopes as described in the earlier question. There is an option to add the encoders for other microscopes with the focus motors. An encoded focus is required for lengthy time lapse experiments where the microscope focus can drift with temperature over time.

1 mm ball screws provide a smaller step size which provides a smoother movement. Compared to 2 mm ballscrew 1 mm will slow the overall movement of the stage down by half. If your requirement is for speed use a 2 or 5 mm ball screw.

400 step motors should be coupled with a 1 mm ball screw to provide an extremely accurate and smooth (under high magnification) stage movement. High-precision encoders are also a good option to further improve this type of stage.

200 step motors are best coupled with 2 mm ballscrews to provide a greater movement speed where the accuracy required is sufficient with the ProScan III standard stage.

Yes, Prior offers a service designing controllers in both small and large quantities. Please see the OEM section of the website for more information, or contact us.

Yes, we offer a service for larger quantities of stages where we will design stages for the purposes of our customers. Please see the OEM section of the website for more information on this, or contact technical support.

Yes, we have an extensive range of sample holders.

If you have a request please contact tech support and supply details of what you would like to hold and which instrument and Prior stage you are using.