PureFocus™850 Laser Autofocus System
Hardware Laser Autofocus For Industrial And Life Science Microscopy
PureFocus850 combines advanced optics and intelligent microprocessing to provide a real-time focus system for infinity-corrected optical systems. It offers sample detection, dynamic power adjustment, and long focus recapture range.
A motorized offset lens allows adjustment of the imaging depth into the sample, continuously holding the precise distance between the imaging focal point and a reference interface of choice or enabling different discrete image planes to be acquired using the same interface.
Mounted in the infinity space, it is compatible with most brands of commercial microscopes as well as Prior’s OpenStand imaging platform, Queensgate piezo devices, and can be adapted for third-party focus actuators. See product compatibility tab for details.
Designed for versatility, PureFocus850 is suitable for applications where it can detect a reflective surface. As well as metallurgical, geological, and semiconductor samples, it can be used for life science applications using slides, glass bottom dishes, and flow chambers.
The laser’s 850 nm operating wavelength does not interfere with common wavelengths used for illumination and imaging.
Users can choose to operate the PureFocus850 as a standalone device or integrate it with control software as part of a comprehensive automated imaging system.
- Rapid automated focus recovery for numerous applications
- Integrated stepper motor driver and interface to Piezo and other actuators via 0-10 V analog output
- Easily adapted to any infinity optical system, both upright and inverted
- Stores and recalls multiple objective-specific parameters for multi-objective use
- Customizable optics for specific imaging applications
| Dichroic clear aperture, diameter | 32 mm |
|---|---|
| Focus accuracy | 20 % of DOF |
| Laser module mass | 1.5 kg |
| Laser optical output | < 0.77 mW |
| Laser class | Class 1 |
| Laser wavelength | 850 nm |
| Surface tracking method | Point on surface tracking |
| Typical update rate | 1 kHz |
| Transmitted wavelength range | 365 – 800 nm |
| Capture speed (5x, 0.13NA) | < 30 ms |
| Capture speed (10x, 0.4NA) | < 50 ms |
| Capture speed (20x, 0.5NA) | < 60 ms |
| Capture speed (40x, 0.95NA) | < 100 ms |
| Capture speed (100x, 0.9NA) | < 1500 ms |
| Capture range (5x, 0.13NA) | +/- 15000 µm |
| Capture range (10x, 0.4NA) | +/- 3000 µm |
| Capture range (20x, 0.5NA) | +/- 1000 µm |
| Capture range (40x, 0.95NA) | +/- 500 µm |
| Capture range (100x, 0.9NA) | +/- 100 µm |
| Dichroic clear aperture, diameter | 32 mm |
| Focus accuracy | 20 % of DOF |
| Laser module mass | 1.5 kg |
| Laser optical output | < 0.77 mW |
| Laser class | Class 1 |
| Laser wavelength | 850 nm |
| Surface tracking method | Averaged surface tracking |
| Typical update rate | 1 kHz |
| Transmitted wavelength range | 365 – 800 nm |
| Capture speed (5x, 0.13NA) | < 30 ms |
| Capture speed (10x, 0.4NA) | < 50 ms |
| Capture speed (20x, 0.5NA) | < 60 ms |
| Capture speed (40x, 0.95NA) | < 100 ms |
| Capture speed (100x, 0.9NA) | < 1500 ms |
| Capture range (5x, 0.13NA) | +/- 15000 µm |
| Capture range (10x, 0.4NA) | +/- 3000 µm |
| Capture range (20x, 0.5NA) | +/- 1000 µm |
| Capture range (40x, 0.95NA) | +/- 500 µm |
| Capture range (100x, 0.9NA) | +/- 100 µm |
- PF209 PureFocus Setup Sample Slide
- PF300 PureFocus Setup Camera
- PF200 PureFocus Setup Target (RMS, W26, M32 threads)
- PF201 PureFocus Setup Target (M25, M27, M32 threads)
- PF404 Piezo Cable for PF850 15D to BNC
PureFocus850-Datasheet.pdf
Nikon-EN-US-Sales-Guide.pdf
Evident-Olympus-EN-US-Sales-Guide.pdf
| Option | Manufacturer | Compatibility | Notes |
|---|---|---|---|
| PF850(M)+ LF335 | Evident/Olympus | BX53M, MX50, MX51, MX61, MX61L, MX63, MX63L | |
| PF850(M)+ LF320 | Nikon | LV100ND, LV100NDA, LV150N, LV150NA, L200N, L200ND, L300N, L300ND, Ni-U | |
| PF850(M)+ LF335 | Prior | OpenStand-U, OpenStand-M, OpenStand-L, OpenStand-V | |
| PF850(M)+ PF208 | Evident/Olympus | IX73 (2-deck) | Contact Prior Scientific if both decks are occupied |
| PF850(M)+ PF214 | Evident/Olympus | IX73 (1-deck) | |
| PF850(M)+ PF203 | Evident/Olympus | IX71 | |
| PF850(M)+ PF213 | Evident/Olympus | IX81 | |
| PF850(M)+ PF211 | Nikon | Ti-U | |
| PF850(M)+ PF212 | Nikon | Ti2-U, Ti2-A | Contact Prior Scientific for Ti2-E |
| PF850(M)+ PF210 | Evident/Olympus | BX51, BX53 | |
| PF850(M)+ LF341 | Zeiss | AxioSkop 40 | Please check with Prior Scientific for Axioimager models |
| PF850(M)+ LF312 | Leica | DM4000, DM5000, DM6000, DM6 |
