Episcopic  Illumination

The OpenStand-V, as is typical for inverted microscopes, features an episcopic illumination pathway with a multi-position turret. This facilitates widefield fluorescence or reflected white light imaging. The path is designed to provide as wide a field of view as possible with commercially available objectives. It can also be adapted to enable laser injection or provide a dual light source input. The accessible frame of the OpenStand-V lends itself to supporting multiple episcopic illumination pathways.

Diascopic Illumination

Diascopic, or transmitted, illumination can also be added to the OpenStand-V, enabling white light critical or Köhler illumination. This is a natural complement to reflected light imaging, for example when using a contrast-based technique to locate cells prior to fluorescence imaging. Prior can support a range of condensers and light sources, and modify its own LED illumination unit, to realize many current and novel applications.

Motorized Stage

The OpenStand-V is designed with Prior’s H117 stages in mind. These stages are well-established platforms for high-content screening, well plate scanning, slide imaging and other inverted microscopy applications. Higher precision and rapid stitching experiments can be achieved by taking advantage of Prior’s encoded and higher-end stage options.

Objective Changer

Prior is constantly developing new motorization hardware for OpenStand products. By motorizing the nosepiece, the correct optics can be moved into the lightpath, with the correct illumination settings, at the right time during an experiment without intervention from the user when supported by appropriate software. OpenStand nosepieces allow fitting of objectives with various thread sizes.

Laser Autofocus

Our PF850 laser autofocus system provides real-time focus control by interfacing with the OpenStand-V focus drive or a piezo-based nanopositioning system, and is suitable for both biological and industrial samples. The autofocus utilizes reflective planes either present in the sample itself or on associated glass interfaces, such as coverslips, to recapture focus.

Camera Mount

Camera mounts enable a variety of cameras to be connected to the microscope for image capture. We offer C-mount, F-mount and T-mount options. The OpenStand-V camera mounts feature a built-in tube lens which can be changed depending on the objectives being used, to optimize image quality and to correct aberrations and magnification errors.