Fluorescence Microscopy techniques are used to study biological events by labeling samples with specific fluorescent probes. These probes emit a known wavelength of light when excited with a different known (typically lower) wavelength of light. The colors in the resulting image help identify specific properties or areas of interest within the sample.

Fluorescence microscopy is a powerful tool for studying the structure and function of cells and tissues. It is used in a wide variety of applications, including:

Cell biology: to study the structure and function of cells, including the distribution of proteins, nucleic acids, and other molecules.

Molecular biology: to examine the interactions between molecules, such as the binding of proteins to DNA or the interaction of enzymes with their substrates.

Immunology: to research the immune system, including the distribution of cells such as lymphocytes and macrophages.

Neuroscience: to see the structure of neurons and the connections between neurons.

Pathology: to diagnose diseases, such as cancer and infection.

Researchers using fluorescence microscopy techniques encounter several obstacles in the process of acquiring good fluorescent images:

• Weak fluorescence signal due to inadequate illumination
• Multiple wavelength studies exposing samples to excessive UV light
• Weak or no signal due to improper sample preparation
• Photobleaching of samples that have been imaged repeatedly

Essential equipment for fluorescent imaging

Prior Scientific’s OpenStand is a fully motorized optical stand that provides the perfect platform for fluorescent imaging with multiple epi-fluorescence options that feature: 

• High-intensity metal halide or LED illumination for strong excitation from ultra-violet to infra-red
• 6 or 8-position high-speed cube turrets to minimize photobleaching while imaging multiple wavelengths
• 350 ms switching time between filter cubes

The OpenStand also minimizes imaging time with fast and precise ProScan XY stages, fast internal motorized Z focus, multiple motorized objective nosepiece options of up to 6 objectives and real-time Laser Autofocus control.

For researchers with existing microscopes Prior is able to provide most of the same motorization to increase the efficiency of any manual fluorescence imaging system, including add-ons:

• ProScan and OptiScan stages
• Filter Wheels for excitation and emission
• High-Speed Shutters
• LED and Metal halide illumination options